Kun Li, Miho Nakajima, Ines Ibañez-Tallon, and Nathaniel Heintz. A cortical circuit for sexually dimorphic oxytocin-dependent anxiety behaviors.Cell, 2016, 167(1): 60–72.e11. doi:10.1016/j.cell.2016.08.067. Presented by Haitao Liu

 

Comments

The authors presented that two pathways in mPFC are coordinated by oxytocin receptor interneurons (OxtrINs) to generate prosocial or anxiolytic behaviors. In the first oxytocin pathway, activation of OxtrINs in the mPFC acts on layer 5 cortical neurons to promote prosocial reaction while causing release of GABA and corticotropin releasing hormone binding protein (CRHBP) that antagonize the excitatory effect of CRH on layer 2/3 cortical neurons through the second CRH pathway. In females, inhibitory effect of the first pro-social behavior pathway is relatively weak because CRH concentration is high, thereby generating higher stress and anxiety. Conversely, the oxytocin pathway in males predominates and CRH concentration is low, in which CRHBP can fully bind to CRH and block its activation of CRH receptor 1 on layer 2/3 pyramidal cells, resulting in anxiolytic effect. This work for the first time highlights the brain interactions between cortical OxtrINs and CRH receptor 1 neurons in neural regulation of social behaviors and represent a very important progress in this research field.

 

Questions discussed:

1. What are the differences between rats and mice in OTR at transcriptional and protein levels?

2. What is the meaning of staying in the central area in open field test?

3. In optogenetic experiment, how to optimize the parameters of blue light stimulation?

4. What is the difference in cytosolic processes between light-evoked firing and spontaneous firing of pyramidal neurons in mPFC?

5. How to explain the difference between female and male mice in the amplitude of evoked current in layer 2/3 pyramidal cells?

6. What determines the amplitude of EPSC and IPSC in patch clamp recording?

7. In cerebral cortex, layer 2 is mainly composed of small pyramidal neurons and numerous stellate neurons and layer 3 contains predominantly small and medium-size pyramidal neurons. Are there any apparent differences between small pyramidal neurons and medium-size neurons in levels of the receptors expression and neurotransmitters?

8. CRH can activate cortical neurons, as the paper mentioned, usually layer 2/3 in mPFC, which are inhibited by activation of OxtrINs. The effect of inhibition is at which types of the cells?

9. The authors mentioned that CRHBP can interact with CRH, so we are curious about the concrete site of CRHBP interaction with CRH?

10. Why did RT-PCR show the gender difference in CRH gene expression (more in paraventricular nucleus in hypothalamus of female mice)?

11. What are the direct or indirect interactions between layer 2/3 and layer 5 pyramidal neurons in mPFC?

12. What are the limitations when we apply OTR agonist and CRHR1 antagonist into clinical therapy?

13. What is the meaning of different EPSC and IPSC amplitude of pyrimidal cells between layer 2/3 and layer 5 in mPFC?


2018年01月19日

2018-1-19 Literature Analysis

Kun Li, Miho Nakajima, Ines Ibañez-Tallon, and Nathaniel Heintz. A cortical circuit for sexually dimorphic oxytocin-dependent anxiety behaviors.Cell, 2016, 167(1): 60–72.e11. doi:10.1016/j.cell.2016.08.067. Presented by Haitao Liu

Add Time:

本网站由阿里云提供云计算及安全服务